Modeling long-term diabetes and related complications in rats J Pharmacol Toxicol Methods.
Mar-Apr ; Epub Nov Gyömrői út Electronic diabetes research erika. DOI: The aims of this study were to establish a model of long-term diabetes with sustained medium scale hyperglycemia and characterize the pathological changes detectable after 4months, with particular respect to dependence on the degree of hyperglycemia.
Methods: Streptozotocin-induced diabetic CFY rats were subjected to four different insulin substitution protocols to achieve different levels of glycemic control Diabetic groups.
Eyes were investigated by ophthalmoscopy, kidney function by urine analysis, and neuropathy by functional tests.
Retinal and renal morphological evaluations were performed by histology, immuno-histochemistry and electron microscopy.
Discussion: Diabetes research established a treatment protocol in rats enabling complex investigation of diabetic retinopathy, nephropathy and neuropathy on a long-term period. Clearly hyperglycemic dependent parameters of these complications serve as good outcome measures for preclinical trials.
Our results provide a useful basis for designing studies for testing preventative diabetes research as well as other translational medical research in this field. Keywords: Anterior segment neovascularization; Chronic model; Cold allodynia; Diabetic nephropathy; Diabetic neuropathy; Diabetic retinopathy; Drug development; Insulin implant; Preclinical model; Streptozotocin. Copyright © Elsevier Inc. All rights reserved.
It found that type 2 diabetes is associated with a higher risk of mortality in hospitalised COVID patients than type 1 diabetes. The combination of an older age and high C-reactive protein CRP was also linked to a higher risk of death. Younger people under 70 years old with chronic kidney disease, a common long-term complication of diabetes, also had a higher likelihood of dying. BMI, however, was not linked to survival.